Action of phospholipase A2 and phospholipase C on Escherichia coli
نویسندگان
چکیده
منابع مشابه
The action of human and rabbit serum phospholipase A2 on Escherichia coli phospholipids.
We have compared the properties of phospholipase A (E.C. 3.1.1.4) activity in whole human and rabbit serum toward the phospholipids of Escherichia coli. Using as substrate E. coli labeled during growth with either [1-(14)C]-palmitic acid or [1-(14)C]oleic acid, and then autoclaved to inactivate E. coli phospholipases and to render the labeled phospholipids accessible to exogenous phospholipases...
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Treatment of dog thyroid slices with an enzyme present in the growth medium of Clostridium perjringens makes them unresponsive to thyroid-stimulating hormone (TSH) as measured by the oxidation of glucose-1J4C to 14C02. This enzyme appears to be phospholipase C since (a) both the anti-TSH effect and the phospholipase activity are inseparable over a 240-fold purification, (b) both activities are ...
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Phospholipases A2 are involved in inflammatory processes such as the liberation of free arachidonic acid from the membrane pool for the biosynthesis of eicosanoids. Inhibitors of these enzymes are proving useful in determining the biological roles of phospholipases A2 in complex cellular processes and may also have therapeutic potential. Inhibition of these lipolytic enzymes is more difficult t...
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BACKGROUND Phospholipases A(2) (PLA(2)) are aggressive enzymes that can destroy phospholipids of cell membranes. The resulting cell fragments trigger the kallikrein-mediated contact phase of coagulation. The aim of the present study was to expose citrated whole blood to PLA(2) and to quantify thrombin generation in recalcified plasma. METHODS Normal citrated blood was exposed to bovine pancre...
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The infection of HeLa cells by poliovirus leads to profound alterations in the activities of both phospholipase C and the A23187-stimulated phospholipase A2. As early as the third hour after poliovirus infection, the activity of phospholipase C is enhanced, as measured by the increase in inositol triphosphate (IP3) in the cells. By the fifth hour post-infection there is a 5-fold increase in IP3...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1974
ISSN: 0003-9861
DOI: 10.1016/0003-9861(74)90176-3